Fig. 3

SLO-MO neuronal activity encodes self-location

(A) Location of neurons with increasing activity following backward (yellow) versus forward (magenta) pre-displacement in example fish.

(B) Examples (top) and average (bottom, with ΔF/F during no-displacement trials subtracted) of neurons with increasing activity following backward pre-displacement. (Shaded regions: SEM in all panels; here, 2 s forward or 1 s backward pre-displacements.)

(C) Examples (top) and averages (bottom) of neurons with increasing activity following forward pre-displacement.

(D) Principal-component analysis embedding of SLO-MO population activity. Trajectories remain separated throughout delay period, gradually converge and return toward the starting point during swim period.

(E) SLO-MO neuron activity across 9 fish (1,347 cell segments) sorted by Spearman correlation, showing consistent correlation to self-location across trial types.

(F and G) For comparison, lack of correlation to self-location for cells with activity correlating to swim vigor, and for cells in pretectum that show visual encoding.

(H) Averages of ΔF/F (centered to zero pre-displacement, normalized in each fish) of positive- and negative-correlating neurons from (E).

(I) Encoding of self-location by SLO-MO neurons during complex trajectories (example fish).

(J) Encoding of sideways changes in self-location in SLO-MO neurons (example fish).