Fig. S5
Positional homeostasis activity and variability, related to Figure 2 (A) Brain activity averaged over trials in an example fish shown as changes over time (ΔF/F)t-1 and (ΔF/F)t. Left: in the 300 ms following onset of backward flow through VR (forward visual motion from the point of view of the fish). Middle: at an intermediate time, after stimulus onset, just before (0–300 ms) swim onset. Right: immediately following (0–300 ms) swim onset. (B) Examples of functional brain maps for individual fish before combining them into the final map in Figure 2D. (C) With a 5 s delay period, fish perform positional homeostasis with little variability. (D and E) Percentage of neurons dropped from each animal was small, and that the majority of these neurons resided in the forebrain. (F and G) With a 17 s delay period, the number of SLO-MO neurons encoding the displacement memory appears to correlate to positional homeostasis performance, although the sample size is too small to be significant. (H) Persistent positional encoding signals were detected in different brain regions, cerebellum, dorsal raphe nucleus, area of the interpeduncular nucleus, habenula, and pallium. |
Reprinted from Cell, 185, Yang, E., Zwart, M.F., James, B., Rubinov, M., Wei, Z., Narayan, S., Vladimirov, N., Mensh, B.D., Fitzgerald, J.E., Ahrens, M.B., A brainstem integrator for self-location memory and positional homeostasis in zebrafish, 50115027.e205011-5027.e20, Copyright (2022) with permission from Elsevier. Full text @ Cell