Fig. 3

Worsened skeletal phenotypes in new nr2f5 mutant. (A) Schematic of nr2f5, with the deletions, RT-PCR primers (rtF and rtR) and functional domains marked. (B) RT-PCR quantification showing similar normalized nr2f5 transcript levels in wild types and nr2f5^ci3000 mutants at 52 hpf. (C-H′) Skeletal preparations of nr2f5 single and combinatorial mutants, visualized as ventral (C-H) and lateral (C′-H′) mounts. Black arrowheads indicate the dysmorphic hyomandibula; brackets highlight missing ceratobranchials; pink arrowheads indicate the transformed upper jaw cartilage. (I) Disrupted arch morphology in double mutants at 30 hpf revealed with the sox10:kikGR transgene. Arches are numbered, and the white dotted line surrounds the first pharyngeal pouch. (J,K) Expression of the mandibular markers dlx5a and dlx6a is expanded into the maxillary prominence (green arrowhead) in nr2f2^ci/ci;nr2f5^ci/ci mutants. Scale bars: 100 µm in C-I; 50 µm in J,K.