PUBLICATION
Control of cranial ectomesenchyme fate by Nr2f nuclear receptors
- Authors
- Okeke, C., Paulding, D., Riedel, A., Paudel, S., Phelan, C., Teng, C.S., Barske, L.
- ID
- ZDB-PUB-221115-47
- Date
- 2022
- Source
- Development (Cambridge, England) 149(23): (Journal)
- Registered Authors
- Barske, Lindsey
- Keywords
- Cranial neural crest, Ectomesenchyme, nr2f2, nr2f5, sox10
- Datasets
- GEO:GSE206903
- MeSH Terms
-
- Animals
- Gene Expression Regulation, Developmental
- Mesoderm
- Neural Crest
- Neural Plate*
- Receptors, Cytoplasmic and Nuclear/genetics
- Zebrafish*/genetics
- PubMed
- 36367707 Full text @ Development
Citation
Okeke, C., Paulding, D., Riedel, A., Paudel, S., Phelan, C., Teng, C.S., Barske, L. (2022) Control of cranial ectomesenchyme fate by Nr2f nuclear receptors. Development (Cambridge, England). 149(23):.
Abstract
Certain cranial neural crest cells are uniquely endowed with the ability to make skeletal cell types otherwise only derived from mesoderm. As these cells migrate into the pharyngeal arches, they downregulate neural crest specifier genes and upregulate so-called ectomesenchyme genes characteristic of skeletal progenitors. While both external and intrinsic factors have been proposed as triggers of this transition, the details remain obscure. Here we report the Nr2f nuclear receptors as novel intrinsic activators of the ectomesenchyme program: zebrafish nr2f5 single and nr2f2;nr2f5 double mutants show marked delays in upregulation of ectomesenchyme genes such as dlx2a, prrx1a/b, sox9a, twist1a, and fli1a, and in downregulation of sox10, which is normally restricted to early neural crest and non-ectomesenchyme lineages. Mutation of sox10 fully rescued skeletal development in nr2f5 single but not nr2f2;nr2f5 double mutants, while the initial ectomesenchyme delay persisted in both. Sox10 perdurance thus antagonizes the recovery but does not explain the impaired ectomesenchyme transition. Unraveling the mechanisms of Nr2f function will help solve the enduring puzzle of how cranial neural crest transition to the skeletal progenitor state.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping