Figure S3

Characterization of jam1a^sd43 zebrafish (related to Figure 3, 4, ,5 and 6) (A, B) Expression of Jam1a in kidney under a kdrl:NTR-cerulean background. A high magnification view of the white dotted area is shown in B. Arrowheads indicate Jam1a expression detected in the sinusoidal endothelium. (C) Mutation in exon 3 of jam1a was verified by sequencing in wild-type orjam1a^sd43 embryos. (D) Schematic diagram of the predicted Jam1a protein in wild-type or jam1a^sd43 animals. Ig, immunoglobulin-like domain; TM, transmembrane domain; PBM, PDZ-binding motif; aa, amino acid. (E, F) Images of wild-type and jam1a^sd43 zebrafish at 4 months of age. (G) Body weights of wild-type and jam1a^sd43 animals at 3 to 6 months of age. Error bar, s.d. (n = 8 (wild-type male), 16 (jam1a^sd43 female), 14 (wild-type female), or 5 (jam1a^sd43 female). (H, L) Expression of Jam1a and GAPDH in wild-type or jam1a^sd43 embryos. (J, K) Expression of Jam1a in the collecting duct (CD) and tubule (CT) of wild-type or jam1a^sd43 animals. (L, M) Expression of runx1 in wild-type or jam1a^sd43 embryos. Arrowheads indicate runx1 expression in the dorsal aorta. (N) Quantitative phenotype distribution of runx1 expression in wild-type 1(2 = 36) or jam1a^sd43 embryos (n = 31). Bars, 10 mm (E, F); 100 μm (L, M); 20 μm (A,J,K); 10 μm (B ). **p ≤ 0.01; ***P < 0.001; n.s. no significance.