FIGURE

Fig. 8

ID

ZDB-FIG-230405-15

Publication

Tan et al., 2022 - Pax2a, Sp5a and Sp5l act downstream of Fgf and Wnt to coordinate sensory-neural patterning in the inner ear

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Fig. 8

Early expression of sp5a and sp5l and effects of disruption on otic patterning. (A) Dorsal views with anterior to the top (Aa-c, Ae-Ag) and dorsal views with anterior to the left (Ad, Ah) showing expression of sp5a and sp5l in wild type embryos at the times indicated at the top. Co-staining for krox20 (red) is shown at 12 hpf (Ab, Af) to mark positions of rhombomeres 3 and 5 (r3, r5) in the hindbrain. The midbrain-hindbrain border (mhb) is labeled. Arrows point to otic domains (Ab, Ac, Af, Ag). (B) Targeting of sp5a and sp5l. The upper diagram represents the general layout of both genes, including regions encoding specific peptide domains. Red arrows show relative positions of deletions induced by CRISPR/Cas9 cutting. Altered sequences in mutant alleles sp5a^x69 and sp5l^x70 are shown in boxed regions and in sequencing traces at the bottom. (C) Lateral views of live wild-type and sp5a−/−; sp5l−/− embryos at 24 hpf. (D) Dorsolateral views of sp5a−/−; sp5l−/− double mutants showing expression at 24 hpf of various otic genes (as indicated on the left) following treatment with DMSO or BIO from 12 hpf (as indicated at the top). Scale bar, 50 μm.

Expression Data

Genes:	atoh1a gsc neurog1 pax2a pou3f3b sp5a sp5l
Fish:	AB sp5a^x69/x69; sp5l^x70/x70
Condition:	chemical treatment by environment: 6-bromoindirubin-3'-oxime
Anatomical Terms:	neural plate otic placode medial region otic vesicle otic vesicle anterior region otic vesicle medial region preplacodal ectoderm
Stage Range:	Bud to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sp5a^x69/x69; sp5l^x70/x70
Condition:	chemical treatment by environment: 6-bromoindirubin-3'-oxime
Observed In:	otic vesicle otic vesicle anterior region otic vesicle medial region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 492, Tan, A.L., Mohanty, S., Guo, J., Lekven, A.C., Riley, B.B., Pax2a, Sp5a and Sp5l act downstream of Fgf and Wnt to coordinate sensory-neural patterning in the inner ear, 139-153, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.