Fig. 2

Effect of LOF Stat5.1 mutation on growth and adiposity. A–D Assessment of adult size and body composition in female (F) and male (M) homozygous stat5.1^wt/wt wild-type (WT) and stat5.1^{mdu022/mdu022} loss-of-function (LOF) mutants at 5 months post-fertilisation (mpf), presenting representative images (A) along with quantification of standard length (B), wet weight (C) and lipid content (D), showing values for individual fish as well as mean ± standard deviation (SD) with statistical significance indicated (n = 6). E–H Growth trajectory during early life, presenting light microscopic images of representative WT and LOF individuals at the 1 cell stage (E) and at the indicated times post-fertilisation (F), along with quantification of length showing mean ± SD and statistical significance (n = 10–33) (G) and relative growth rate in the indicated age ranges calculated from these data with p-values shown (H). Scale bar = 1 mm. I–J Lipid analysis in juveniles, presenting fluorescent images of representative 14 dpf WT and LOF stained with Nile Red (I) and quantification of the area of Nile Red staining showing individual values as well as mean ± SD, with statistical significance indicated (n = 24) (J). Scale bar = 1 mm. K Expression analysis of growth and metabolic genes in 28 dpf juveniles (K) using qRT²-PCR on WT and LOF samples. Data for the indicated genes were normalised to actb and represented as relative fold-change compared to WT, with mean ± SD shown and statistical significance indicated (n = 6)