Figure 3

Effect of socs3b ablation on early definitive hematopoiesis. Representative images of wildtype (WT, socs3b^wt/wt) and knockout (KO, socs3b^mdu24/mdu24) embryos subjected to WISH using mpx(A, B), lcp1(C, D), csf3r(G, H), mpeg1.1(J, K), hbbe1.1(M, N) and rag1(P, Q) at 5 dpf, showing the major areas of staining in each case, with scale bars representing 200 μm. Fluorescent images of WT and KO embryos on either the Tg(mpx::GFP) (S, T) or Tg(mpeg1.1::GFP) (V, W) transgenic backgrounds, with scale bars representing 200 μm. Quantitation of mpx⁺(E), lcp1⁺(F), csf3r⁺(I), mpeg1.1⁺(L) and GFP⁺ cells in Tg(mpx::GFP) (U) and Tg(mpeg1.1::GFP) (X) embryos, and area of staining for hbbe1.1(O) and rag1(R) showing values for individual embryos along with mean and SEM, with statistical significance indicated. Student t-tests were used to measure the significance between WT and KO with Welch’s correction performed if required (*** p < 0.001, ** p < 0.01).