PUBLICATION
Socs3b regulates the development and function of innate immune cells in zebrafish
- Authors
- Sobah, M.L., Scott, A.C., Laird, M., Koole, C., Liongue, C., Ward, A.C.
- ID
- ZDB-PUB-230329-35
- Date
- 2023
- Source
- Frontiers in immunology 14: 11197271119727 (Journal)
- Registered Authors
- Liongue, Clifford, Ward, Alister C.
- Keywords
- cytokine signaling, inflammation, innate immunity, socs3, zebrafish
- MeSH Terms
-
- Animals
- Immunity, Innate
- Macrophages
- Signal Transduction
- Suppressor of Cytokine Signaling Proteins*/metabolism
- Zebrafish*
- PubMed
- 36969252 Full text @ Front Immunol
Citation
Sobah, M.L., Scott, A.C., Laird, M., Koole, C., Liongue, C., Ward, A.C. (2023) Socs3b regulates the development and function of innate immune cells in zebrafish. Frontiers in immunology. 14:11197271119727.
Abstract
Introduction Suppressor of cytokine signaling 3 (SOCS3) is a critical component of the negative feedback regulation that controls signaling by cytokines and other factors thereby ensuring that important processes such as hematopoiesis and inflammation occur at appropriate levels.
Methods To gain further insights into SOCS3 function, the zebrafish socs3b gene was investigated through analysis of a knockout line generated using CRISPR/Cas9-mediated genome editing.
Results Zebrafish socs3b knockout embryos displayed elevated numbers of neutrophils during primitive and definitive hematopoiesis but macrophage numbers were not altered. However, the absence of socs3b reduced neutrophil functionality but enhanced macrophage responses. Adult socs3b knockout zebrafish displayed reduced survival that correlated with an eye pathology involving extensive infiltration of neutrophils and macrophages along with immune cell dysregulation in other tissues.
Discussion These findings identify a conserved role for Socs3b in the regulation of neutrophil production and macrophage activation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping