FIGURE

Figure 4

ID

ZDB-FIG-230228-122

Publication

Ritter et al., 2023 - Rgp1 contributes to craniofacial cartilage development and Rab8a-mediated collagen II secretion

Other Figures

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Figure 4

Collagen II accumulations in rgp1^-/- chondrocytes localize to endolysosomes (A) TEM images of 3 dpf WT craniofacial chondrocytes. (B) TEM images of 3 dpf rgp1^-/- craniofacial chondrocytes showing vacuolar structures (yellow arrowhead) (B’) Enlarged image of boxed area showing striated ultrastructure of intracellular collagen fibrils (yellow arrowhead). (B”) Further enlargement of boxed area. (C) Representative images of chondrocytes in WT and rgp1^-/- cartilage (CHP, magenta; LBPA, green). Insets are enlargements of white dashed boxes, and an intracellular accumulation is labeled with a yellow arrowhead. (D) Quantification of the Pearson R value for co-localization in individual chondrocytes. Significance is presented by ****p<0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rgp1^vmc4/vmc4; vmc7Tg (AB) rgp1^vmc5/vmc5; vmc7Tg (AB) rgp1^vmc6/vmc6; vmc7Tg (AB)
Observed In:	chondrocyte collagen type II trimer chondrocyte endolysosome
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)