FIGURE

Figure 1

ID

ZDB-FIG-230228-118

Publication

Ritter et al., 2023 - Rgp1 contributes to craniofacial cartilage development and Rab8a-mediated collagen II secretion

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Figure 1

Rgp1 is required for zebrafish craniofacial skeletal development. (A) Zebrafish rgp1 intron/exon map showing locations of g1 and g3 gRNA target sites. (B) Sequences from three different alleles isolated from the use of g1 and g3 gRNAs. (C) Predicted peptide lengths for alleles shown in (B). (D) Live imaging of 5 dpf WT and rgp1^-/- larvae from ventral and lateral view showing jaw protrusions (black arrowhead). (E) Alcian blue staining and imaging of 5 dpf WT and rgp1^-/- cartilage in the ventral craniofacial head skeleton. The Meckel’s cartilage (red), and ceratohyal (yellow, dashed) are marked. (F) Quantification of larva head length. (G) Quantification of Meckel’s cartilage length. (H) Quantification of ceratohyal cartilage angle. (I) Schematic for zebrafish rgp1 mRNA injection and rescue experiment. (J) Lateral images of larvae showing jaw protrusion, partial protrusion, and no protrusion phenotypes (black arrowheads). (K) Quantification of jaw phenotype percentages in groups of injected and non-injected embryos. Significance is presented by ****p<0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rgp1^vmc4/vmc4 (AB) rgp1^vmc5/vmc5 (AB) rgp1^vmc6/vmc6 (AB)
Observed In:	ceratohyal cartilage head mandibular arch skeleton Meckel's cartilage
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)