FIGURE

Figure 3

ID

ZDB-FIG-230228-121

Publication

Ritter et al., 2023 - Rgp1 contributes to craniofacial cartilage development and Rab8a-mediated collagen II secretion

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Figure 3

Rgp1^-/- chondrocytes accumulate collagen II intracellularly. (A) Experimental design for immunohistochemical analysis of chondrocytes in the craniofacial head skeleton. (B) Representative images of Tg(Col2a1α:caax-eGFP) transgenic larvae in WT and rgp1^-/- cartilage (Col2, magenta; eGFP, green). Yellow arrowhead identifies intracellular collagen II. (C) Quantification of the percentage of cytosolic area occupied by collagen II signal in chondrocytes. (D) Experimental design for mosaic overexpression of zRgp1-p2A-mCherry in zebrafish chondrocytes. (E) Representative images of Tg(Col2a1α:caax-eGFP) transgenic chondrocytes in WT and rgp1^-/- cartilage (TEM images of chondrocyte membranes (PM) and adjacent mCherry, magenta; eGFP, green; Col2, white). Yellow arrowhead identifies collagen II in mCherry^- cells while empty arrowhead identifies collagen II in mCherry⁺ cells. (F) Quantification of the percentage of cytosolic area occupied by collagen II signal in chondrocytes. (G) TEM images of chondrocyte membranes and adjacent ECM proteins. (H) Quantification of TEM staining density from craniofacial chondrocyte plasma membrane to edge of cartilage tissue using ImageJ. Significance is presented by ****p<0.0001; ns, not signficant.

Expression Data

Antibody:	Ab1-col2a
Fish:	vmc7Tg (AB) rgp1^vmc4/vmc4; vmc7Tg (AB) rgp1^vmc5/vmc5; vmc7Tg (AB) rgp1^vmc6/vmc6; vmc7Tg (AB)
Anatomical Terms:	chondrocyte cytoplasm chondrocyte extracellular space
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rgp1^vmc4/vmc4; vmc7Tg (AB) rgp1^vmc5/vmc5; vmc7Tg (AB) rgp1^vmc6/vmc6; vmc7Tg (AB)
Observed In:	chondrocyte cytoplasm chondrocyte extracellular space
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)