FIGURE

Fig. 5

ID

ZDB-FIG-230114-26

Publication

Katraki-Pavlou et al., 2021 - Protein tyrosine phosphatase receptor zeta 1 deletion triggers defective heart morphogenesis in mice and zebrafish

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Fig. 5

Expression of cardiac markers during zebrafish cardiac development. A–F: expression of cardiac genes using whole mount antisense ISH. The boundaries of the heart are drawn with dashed lines, black arrowheads indicate the location of the atrioventricular valves (AV), whereas white arrowheads indicate the outflow tract (OFT). Scale bars correspond to 100 μm. G–I: T-box family genes relative expressions. J–L: cardiac development regulator genes relative expressions. For G–L, RNA was isolated from ptprz1b^+/+ and ptprz1b^−/− embryos at 3 and 5 dpf and mRNA levels were determined by qRT-PCR and calculated by the 2^ΔCT method. Results are expressed as means ± SD (n = 4) and statistical analysis was performed by Student’s unpaired t test. hpf, hours postfertilization; ISH, in situ hybridization; OFT, outflow tract; A, atrium; V, ventricle; AV, atrioventricular canal.

Expression Data

Genes:	bmp4 gata4 isl1a klf2a notch1b tbx2a tbx2b tbx18 tbx20
Fish:	ia5Tg; s843Tg (AB) ptprz1b^aa65/aa65; ia5Tg; s843Tg (AB)
Anatomical Terms:	atrioventricular canal cardiac ventricle whole organism
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ptprz1b^aa65/aa65; ia5Tg; s843Tg (AB)
Observed In:	atrioventricular canal cardiac ventricle whole organism
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Heart Circ. Physiol.