ZFIN Image: Katraki-Pavlou et al., 2021, Fig. 5

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Fig. 5

Expression of cardiac markers during zebrafish cardiac development. A–F: expression of cardiac genes using whole mount antisense ISH. The boundaries of the heart are drawn with dashed lines, black arrowheads indicate the location of the atrioventricular valves (AV), whereas white arrowheads indicate the outflow tract (OFT). Scale bars correspond to 100 μm. G–I: T-box family genes relative expressions. J–L: cardiac development regulator genes relative expressions. For G–L, RNA was isolated from ptprz1b^+/+ and ptprz1b^−/− embryos at 3 and 5 dpf and mRNA levels were determined by qRT-PCR and calculated by the 2^ΔCT method. Results are expressed as means ± SD (n = 4) and statistical analysis was performed by Student’s unpaired t test. hpf, hours postfertilization; ISH, in situ hybridization; OFT, outflow tract; A, atrium; V, ventricle; AV, atrioventricular canal.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Heart Circ. Physiol.