Figure 5

Loss of gtpbp1l reduced the expression of vessel-related markers. (A,C,E,G,I) Whole-mount in situ hybridization in wild-type (wt) controls and in gtpbp1l^atg morphants (B,D,F,H,J). At 24 hpf, gtpbp1l^atg morphants showed decreased expression (dash lines) of the arterial marker ephrinb2 (B), venous markers flt4 (D) and mrc1 (F), and pan-vascular markers flk (H) and stabilin (J) compared to controls. Dorsal aorta (a); vein (v); vessel (Ve); intersegmental vessels (isv), and caudal vein plexus (CVP). (K) Quantitative qPCR analysis revealed the relative expression levels of ephrinb2 (0.63 ± 0.22), flt4 (0.60 ± 0.22), mrc1(0.74 ± 0.25), flk1(0.63 ± 0.20), and stabilin (0.54 ± 0.12) in gtpbp1l^atg morphants, which is normalized to wt controls. Values on the bottom right indicate the number of embryos exhibiting the staining pattern per total number. Data are mean ± S.D. *** p < 0.0001, ** p < 0.001, and * p < 0.05 according to unpaired Student’s t-test. Scale bars are 200 µm.