Fig. 5
- ID
- ZDB-FIG-221222-12
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- Chen et al., 2021 - Acute brain vascular regeneration occurs via lymphatic transdifferentiation
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Figure 5. EphB4a represses track iLV transdifferentiation through suppression of Notch (A–C) The derepressed transdifferentiation of track iLV (Dendra2+DsRed+) in the ephB4a mutant (A, arrow) was rescued by treatment with DAPT, which returned to Dendra2−DsRed+ (B, arrowheads). The statistics show the ratios of transdifferentiation among track iLVs in the ephB4a mutant with or without DAPT treatment (C) (n = 10 larvae; two-tailed unpaired t test; ∗∗∗, p < 0.0001). (D–G) The derepressed transdifferentiation of track iLV (Dendra2+GFP+) in the ephB4a mutant without heat shock (D, arrow) or without dnMAML-Flag (E, arrow) was rescued by the heat-shock-induced, LEC-specific overexpression of dnMAML-Flag, which exhibited Dendra2−GFP+ at 4 dpt (F, arrowhead). The statistics show the ratios of transdifferentiation among track iLVs in the ephB4a mutant groups (G) (n = 10 larvae; two-tailed unpaired t test; ∗∗∗, p < 0.0001). Scale bar, 20 μm. Data are represented as mean ± SD. HS, heat shock. See also Figures S6 and S7. |
Reprinted from Developmental Cell, 56(22), Chen, J., Li, X., Ni, R., Chen, Q., Yang, Q., He, J., Luo, L., Acute brain vascular regeneration occurs via lymphatic transdifferentiation, 3115-3127.e6, Copyright (2021) with permission from Elsevier. Full text @ Dev. Cell