Fig 12
Phenotype analysis of βA and βB double mutants.
Double mutants involving both βA and βB subunits were created (inhbaa-/-;inhbb-/-, and inhbab-/-;inhbb-/-) and their ovaries examined at different time points (100–270 dpf). (A) Histological analysis of inhbaa-/-;inhbb-/- from 100 to 240 dpf. (B) Follicle composition in the ovaries of the inhbaa;inhbb mutants at 165 dpf (*** p < 0.001, n = 4). (C) Histological analysis of inhbab-/-;inhbb-/- from 100 to 270 dpf. (D) Follicle composition in the ovaries of the inhbab;inhbb mutants at 270 dpf (* p < 0.05; ** p < 0.01, n = 4). No interactive effects were observed between βA and βB in terms of histological structure and follicle composition. The double mutants phenocopied the single mutants inhbaa-/- and inhbb-/-, respectively, showing similar ovarian disorganization, follicle degeneration, and accumulation of somatic stromal cells (asterisk) and fluid (arrowhead) at 165 and 240 dpf for inhbaa-/-;inhbb-/- and 270 dpf for inhbab-/-;inhbb-/-. The numbers shown in the photos indicate the total number of fish examined (lower) and the fish exhibiting similar phenotype to that shown (upper). PV, pre-vitellogenic; MV, mid-vitellogenic; LV, late vitellogenic; FG, full-grown. |