Opsin mis-localization was found in pomt2 mutant retinas. Retinal sections from zebrafish at 6-mpf were immunostained with antibodies 1D4 and 4D2 (red fluorescence) and counter-stained with DAPI (blue) (n = 3). (A) 1D4 immunostaining of a wild-type retina at 6-mpf. (B) 1D4 immunostaining of a homozygous pomt2sny5+13 mutant retina at 6-mpf. Note the presence of strongly 1D4-stained cell bodies (arrowhead) and terminals (arrow). (C) 4D2 immunostaining of a wild-type retina at 6-mpf. (D) 4D2 immunostaining of a homozygous pomt2sny5+13 mutant retina at 6-mpf. Note strongly 4D2 fluorescent cell bodies in the ONL. Scale bar in (D): 10 µm.
|