FIGURE

Fig. 5

ID

ZDB-FIG-221207-17

Publication

Jia et al., 2022 - Tulp1 deficiency causes early-onset retinal degeneration through affecting ciliogenesis and activating ferroptosis in zebrafish

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Fig. 5

Ferroptosis signaling pathway was activated in tulp1-dKO zebrafish.

A KEGG pathway enrichment analysis of the genes identified to be differentially expressed between wt and tulp1-dKO groups. The significantly enriched pathways are shown. B GSEA of ferroptosis-related genes. C–H Relative expression of genes involved in ferroptosis was detected by qRT-PCR at 4 dpf. Mean ± SD. *P < 0.05. **P < 0.01. Lipid droplets performed by Nile Red (I) or BODIPY (J) in wt and tulp1-dKO zebrafish at 4 dpf. Enlarged images of the boxed areas are shown in the lower right corner. Scale bar: 20 µm. K Perls/DAB staining in wt and tulp1-dKO zebrafish at 4 dpf. Enlarged images of the boxed areas are shown beneath. Scale bar: 25 µm. L TEM images of mitochondria from wt and tulp1-dKO zebrafish. M, mitochondria. OS, outer segment. Scale bar: 0.2 µm.

Expression Data

Genes:	alox5b.3 cp cyp2p9 cyp2p10 ptgs2b fthl28
Fish:	WT tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19
Anatomical Term:	head
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19
Observed In:	eye photoreceptor cell ferroptosis eye photoreceptor cell intracellular iron ion homeostasis eye photoreceptor cell iron cation eye photoreceptor cell mitochondrial crista eye photoreceptor cell mitochondrial membrane head retina retina lipid
Stage:	Day 4

Phenotype Detail

Acknowledgments

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