FIGURE

Fig. 6

ID

ZDB-FIG-221123-36

Publication

Sarvari et al., 2021 - The E3 ubiquitin-protein ligase Rbx1 regulates cardiac wall morphogenesis in zebrafish

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Fig. 6

Pharmacological activation of Hedgehog signaling normalizes the multi-layered myocardial wall phenotype in rbx1 mutants

(A-D) Confocal images (mid-sagittal sections) of 102 hpf hearts from Tg(myl7:EGFP-Hsa.HRAS) rbx1^+/− incrosses. Sagittal views of the ventricle from rbx1^+/+ (A–B) or rbx1^−/− (C–D) siblings treated with DMSO (A, C) or 10 μM of the hedgehog activator drug (SAG) (B, D) from 48 to 96 hpf. (E–F) Confocal images (mid-sagittal sections) of 102 hpf hearts from Tg(myl7:EGFP-Hsa.HRAS) rbx1^+/+ animals treated with ethanol (E) or 10 μM of the hedgehog inhibitor drug (Cyclopamine) from 48 to 96 hpf (F). (G–H) Percentage of inner layer CMs relative to the total number of CMs after DMSO or SAG treatment in rbx1^+/+ (G) and rbx1^−/− (H) larvae. (I) Percentage of inner layer CMs relative to the total number of CMs after ethanol or Cyclopamine treatment in rbx1^+/+ animals. Each dot represents one heart. Data are shown as mean ± SEM. P-values calculated by Student's t-test. V: ventricle, A: atrium; scale bars, 50 μm.

Expression Data

Gene:	EGFP
Fish:	s883Tg rbx1^{bns148/bns148}; s883Tg
Conditions:	chemical treatment by environment: Cyclopamine chemical treatment by environment: 3-chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[3-(pyridin-4-yl)benzyl]-1-benzothiophene-2-carboxamide
Anatomical Term:	ventricular myocardium
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s883Tg rbx1^{bns148/bns148}; s883Tg
Conditions:	chemical treatment by environment: Cyclopamine chemical treatment by environment: 3-chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[3-(pyridin-4-yl)benzyl]-1-benzothiophene-2-carboxamide
Observed In:	cardiac muscle cell ventricular myocardium ventricular myocardium central region
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 480, Sarvari, P., Rasouli, S.J., Allanki, S., Stone, O.A., Sokol, A., Graumann, J., Stainier, D.Y.R., The E3 ubiquitin-protein ligase Rbx1 regulates cardiac wall morphogenesis in zebrafish, 1-12, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.