FIGURE

Fig. 5

ID

ZDB-FIG-221123-35

Publication

Sarvari et al., 2021 - The E3 ubiquitin-protein ligase Rbx1 regulates cardiac wall morphogenesis in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

rbx1 overexpression in endothelial cells can rescue the multi-layered myocardial wall phenotype in rbx1 mutants

(A-B) Cartoon of rbx1 overexpression transgene constructs under myocardial-specific (A) or endothelial-specific (B) promoters. (C–C′) Confocal images (mid-sagittal sections) of 96 hpf Tg(myl7:EGFP-Hsa.HRAS) rbx1^+/+ (C) and rbx1^−/− (C′) hearts. (D-E′) Overexpression of rbx1 in the endothelium but not the myocardium can rescue the myocardial wall phenotype in rbx1^−/− larvae. (D-D′) Confocal images (mid-sagittal sections) of 96 hpf rbx1^+/+ (D) and rbx1^−/− (D′) hearts in the presence of the myl7:rbx1-p2a-tdTomato transgene. (E-E′) Confocal images (mid-sagittal sections) of 96 hpf rbx1^+/+ (E) and rbx1^−/− (E′) hearts in the presence of the fli1a:rbx1-p2a-tdTomato transgene. Magnified views of dashed areas shown in the right corners. (C"-E") Percentage of inner layer CMs relative to the total number of CMs comparing rbx1^+/+ and rbx1^−/− hearts having no rbx1 overexpression (C'’), and with overexpression of rbx1 in the myocardium (D'’) or endocardium (E'’). V: ventricle, A: atrium; scale bars, 50 μm.

Expression Data

Genes:	EGFP Tomato
Fish:	s883Tg bns236Tg; s883Tg bns237Tg; s883Tg rbx1^{bns148/bns148}; s883Tg rbx1^{bns148/bns148}; bns236Tg; s883Tg rbx1^{bns148/bns148}; bns237Tg; s883Tg
Anatomical Terms:	ventricular endocardium ventricular myocardium
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rbx1^{bns148/bns148}; s883Tg rbx1^{bns148/bns148}; bns236Tg; s883Tg rbx1^{bns148/bns148}; bns237Tg; s883Tg
Observed In:	cardiac muscle cell ventricular myocardium ventricular myocardium central region
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 480, Sarvari, P., Rasouli, S.J., Allanki, S., Stone, O.A., Sokol, A., Graumann, J., Stainier, D.Y.R., The E3 ubiquitin-protein ligase Rbx1 regulates cardiac wall morphogenesis in zebrafish, 1-12, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.