FIGURE

Figure 7

ID

ZDB-FIG-221118-123

Publication

Sebo et al., 2022 - Functional identification of the zebrafish Interleukin-1 receptor in an embryonic model of Il-1β-induced systemic inflammation

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Figure 7

Analysis of Reactive Oxygen Species in Il-1β-induced inflammation. (A) Representative confocal images of ubb:Gal4-EcR, uas:Ilβ^mat larvae treated with CM-H₂DCFDA. Shown here are the overlay of brightfield and CM-H₂DCFDA fluorescence (left panels) and CM-H₂DCFDA fluorescence only (right panels). Fluorescence signal was quantified from the region of interest outlined with a white border in the CM-H₂DCFDA images. (B) Relative Fluorescence Intensity (RFI) was quantified using FIJI ‘mean grey value’ measurement. All mean RFIs were normalized to uninjected No Teb mean values. Error Bars are +1 standard deviation. Asterisks indicate significant differences **p < 0.01 ***p < 0.001 by one-way ANOVA followed by Tukey’s HSD Test. Scale bar in (A) is 1 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	uw110Tg; uw111Tg (AB) uw110Tg; uw111Tg + MO1-il1rl2 (AB) uw110Tg; uw111Tg + MO1-zmp:0000000936 (AB)
Condition:	chemical treatment by environment: tebufenozide
Knockdown Reagents:	MO1-il1rl2 MO1-zmp:0000000936
Observed In:	whole organism reactive oxygen species
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol