ZFIN Image: Sebo et al., 2022, Figure 7

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Figure Caption

Figure 7

Analysis of Reactive Oxygen Species in Il-1β-induced inflammation. (A) Representative confocal images of ubb:Gal4-EcR, uas:Ilβ^mat larvae treated with CM-H₂DCFDA. Shown here are the overlay of brightfield and CM-H₂DCFDA fluorescence (left panels) and CM-H₂DCFDA fluorescence only (right panels). Fluorescence signal was quantified from the region of interest outlined with a white border in the CM-H₂DCFDA images. (B) Relative Fluorescence Intensity (RFI) was quantified using FIJI ‘mean grey value’ measurement. All mean RFIs were normalized to uninjected No Teb mean values. Error Bars are +1 standard deviation. Asterisks indicate significant differences **p < 0.01 ***p < 0.001 by one-way ANOVA followed by Tukey’s HSD Test. Scale bar in (A) is 1 mm.

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Acknowledgments

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