Figure 3

ER stress response in 5 dpf wfs1ab^KO zebrafish larvae under physiological and ER stress conditions after tunicamycin treatment

(A) mRNA levels of ER stress markers were analyzed by qPCR and (B) protein levels by western blot in wfs1ab^WT and wfs1ab^KO larvae. (C) Pictures of typical blots (completely stain free) are shown in Figure S4. (D) Larvae were exposed to vehicle DMSO solution (Veh) or tunicamycin 2 μg/mL (Tunica) for 24 h. zeif2α and stain-free were used as a loading control in qPCR and western blot analyses, respectively. The relative expression levels of mRNA after tunicamycin treatment are indicated as the fold change from the respective wfs1ab^WT/DMSO or wfs1ab^KO/DMSO control group, when higher than 2-fold. Data are expressed as mean ± SEM, n = 5–9 in each group. ∗p < 0.05 versus wfs1ab^WT in (A and B). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus DMSO treatment in (A and B) and Tukey’s multiple comparison test in (D).