FIGURE

Figure 2

ID

ZDB-FIG-221104-54

Publication

Crouzier et al., 2022 - NCS1 overexpression restored mitochondrial activity and behavioral alterations in a zebrafish model of Wolfram syndrome

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Figure 2

Morphological characterization of the retina and glycemia analysis of the wfs1ab^KO zebrafish line

(A) Typical micrographs of the retina, (B) quantification of the number of ganglion cells, and (C) quantification of the thickness of the associated layer. Confocal images were obtained from sections from wfs1ab^WT and wfs1ab^KO zebrafish retina, showing cell nuclei labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). (D and E) (D) Typical micrographs of the cones (green) and (E) quantification of photoreceptor cells (red and green cones) labeled with Zpr-1 antibody. (F and G) (F) Typical micrographs of the rods (red) and (G) quantification of rods labeled with Rho4d2 antibody. (H) Pancreas characterization with measurement of blood glucose on whole larvae. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Scale bars, 50 μm in (A, D, and F). The number of animals is indicated in the columns. ∗p < 0.05; unpaired t test.

Expression Data

Antibodies:	Ab5-rho zpr-1
Fish:	WT wfs1a^{sa11465/sa11465}; wfs1b^{sa16422/sa16422}
Anatomical Terms:	long double cone cell retinal rod cell short double cone cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wfs1a^{sa11465/sa11465}; wfs1b^{sa16422/sa16422}
Observed In:	long double cone cell retinal rod cell short double cone cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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