FIGURE

Fig. 9

ID

ZDB-FIG-221030-79

Publication

Han et al., 2021 - Loss of smarcad1a accelerates tumorigenesis of malignant peripheral nerve sheath tumors in zebrafish

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Fig. 9

Human cells show a defect in double-strand DNA damage repair. (A, B) Double-strand DNA damage repair was measured by Western blots with anti-histone H2AX S139ph (phospho Ser139) in SMARCAD1 knockdown schwannoma cell line HEI-193 after X-ray irradiations (10 and 20 Gy, respectively). ULTRA-3351712 knockdown cell was used for this experiment. Doxycycline (final concentration: 0.1 μg/ml) was added 48 h ahead of the experiments. (C, D) Double-strand DNA damage repair in SMARCAD1 overexpression MPNST cells (STS26T) by H2AX S139ph. Doxycycline (final concentration: 1 μg/ml) was added 24 h ahead of the experiments. Both cell lines were harvested at 1, 5, and 10 h post-irradiation along with untreated samples. un, untreated sample. Beta-actin (ACTB) was used as a loading control. The densitometry ratios of H2A X S139ph over ACTB was listed underneath the blots

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Genes Chromosomes Cancer