PUBLICATION
Loss of smarcad1a accelerates tumorigenesis of malignant peripheral nerve sheath tumors in zebrafish
- Authors
- Han, H., Jiang, G., Kumari, R., Silic, M.R., Owens, J.L., Hu, C.D., Mittal, S.K., Zhang, G.J.
- ID
- ZDB-PUB-210729-7
- Date
- 2021
- Source
- Genes, chromosomes & cancer 60(11): 743-761 (Journal)
- Registered Authors
- Zhang, GuangJun
- Keywords
- CRISPR, MPNST, SMARCAD1, smarcad1a, tp53 DNA damage repair, zebrafish
- MeSH Terms
-
- Animals
- Carcinogenesis/genetics*
- Carcinogenesis/metabolism
- DNA Breaks, Double-Stranded
- DNA Repair
- Neurofibrosarcoma/genetics*
- Neurofibrosarcoma/metabolism
- Tumor Suppressor Protein p53/genetics
- Tumor Suppressor Protein p53/metabolism
- Zebrafish
- PubMed
- 34296799 Full text @ Genes Chromosomes Cancer
Citation
Han, H., Jiang, G., Kumari, R., Silic, M.R., Owens, J.L., Hu, C.D., Mittal, S.K., Zhang, G.J. (2021) Loss of smarcad1a accelerates tumorigenesis of malignant peripheral nerve sheath tumors in zebrafish. Genes, chromosomes & cancer. 60(11):743-761.
Abstract
Malignant peripheral nerve sheath tumors (MPNSTs) are a type of sarcoma that generally originates from Schwann cells. The prognosis for this type of malignancy is relatively poor due to complicated genetic alterations and the lack of specific targeted therapy. Chromosome fragment 4q22-23 is frequently deleted in MPNSTs and other human tumors, suggesting tumor suppressor genes may reside in this region. Here, we provide evidence that SMARCAD1, a known chromatin remodeler, is a novel tumor suppressor gene located in 4q22-23. We identified two human homologous smarcad1 genes (smarcad1a and smarcad1b) in zebrafish, and both genes share overlapping expression patterns during embryonic development. We demonstrated that two smarcad1a loss-of-function mutants, sa1299 and p403, can accelerate MPNST tumorigenesis in the tp53 mutant background, suggesting smarcad1a is a bona fide tumor suppressor gene for MPNSTs. Moreover, we found that DNA double-strand break (DSB) repair might be compromised in both mutants compared to wildtype zebrafish, as indicated by pH2AX, a DNA DSB marker. In addition, both SMARCAD1 gene knockdown and overexpression in human cells were able to inhibit tumor growth and displayed similar DSB repair responses, suggesting proper gene expression level or gene dosage is critical for cell growth. Given that mutations of SMARCAD1 sensitize cells to PARP inhibitors in yeast and the human U2OS osteosarcoma cell line, the identification of SMARCAD1 as a novel tumor suppressor gene might contribute to the development of new cancer therapies for MPNSTs. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping