FIGURE

Fig. 1

ID

ZDB-FIG-221028-16

Publication

Cacialli et al., 2022 - Synergistic prostaglandin E synthesis by myeloid and endothelial cells promotes fetal hematopoietic stem cell expansion in vertebrates

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Fig. 1

The PGE₂ synthesis pathway in zebrafish CHT

A
Experimental outline of qPCR analysis after dissection of CHT (caudal region) at 48 hpf from kdrl:GFP, ikaros:GFP, mpeg1:GFP, mpx:GFP transgenic animals, and FACS‐sorted GFP‐positive cells to purify ECs, HSPCs, macrophages, and neutrophils.
B, C
The phospholipases pla2g4aa and pla2g4ab are highly expressed in neutrophils.
D–F
The expression of cyclooxygenases cox1, cox2a, and cox2b is enriched in macrophages.
G, H
The prostaglandin synthases ptges3a and ptges3b are only expressed by ECs.
I–M
The prostaglandin receptors ptger1a, ptger1b, ptger2a, ptger3, and ptger4a are mostly expressed in HSPCs.
N, O
The prostaglandin transporters slco2b1 and abcc4 are specifically expressed in ECs.

Data information: For each panel, data represent biological triplicates plated in technical duplicates. Statistical analysis was completed using one‐way ANOVA and multiple comparison tests. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Center values denote the mean, and error bars denote s.e.m.

Source data are available online for this figure.

Expression Data

Genes:	abcc4 EGFP GFP mt-co1 pla2g4aa pla2g4ab ptger1a ptger1b ptger2a ptger3 ptger4a ptges3a ptges3b ptgs2a ptgs2b slco2b1
Fish:	fr101Tg/fr101Tg gl22Tg/gl22Tg i113Tg/i113Tg s843Tg/s843Tg
Anatomical Terms:	endothelial cell hematopoietic multipotent progenitor cell macrophage neutrophil
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fr101Tg/fr101Tg gl22Tg/gl22Tg i113Tg/i113Tg
Observed In:	macrophage neutrophil
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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