FIGURE

Fig. 2

ID

ZDB-FIG-221028-17

Publication

Cacialli et al., 2022 - Synergistic prostaglandin E synthesis by myeloid and endothelial cells promotes fetal hematopoietic stem cell expansion in vertebrates

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Fig. 2

The loss of HSCs after myeloid ablation can be rescued by PGE2 or PGH2 treatments

A
Schematic indicating the imaging area in the tail at 72 hpf, as indicated by the black box; fluorescence imaging of the CHT in double transgenic cd45:CFP‐NTR/runx1:mcherry embryos in DMSO and after treatment with MTZ and PGH2 or PGE2_.
B
Quantification of runx1:mcherry‐positive cells in double transgenic cd45:CFP‐NTR/runx1:mcherry embryos in DMSO and after treatment with MTZ and PGH2 or PGE2_.
C
Quantification of cd45:CFP‐positive cells in double transgenic cd45:CFP‐NTR/runx1:mcherry embryos in DMSO and after treatment with MTZ and PGH2 or PGE2_.

Data information: Statistical analysis was completed using one‐way ANOVA and multiple comparison tests. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Scale bar is 200 μm (A).

Source data are available online for this figure.

Expression Data

Genes:	CFP mCherry
Fish:	cz2010Tg/cz2010Tg; ug108Tg/ug108Tg
Conditions:	chemical treatment by environment: dimethyl sulfoxide chemical treatment by environment: metronidazole chemical treatment by environment: metronidazole chemical treatment by environment: metronidazole chemical treatment by environment: prostaglandin E2 chemical treatment by environment: prostaglandin H2
Anatomical Term:	myeloid cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cz2010Tg/cz2010Tg; ug108Tg/ug108Tg
Conditions:	chemical treatment by environment: metronidazole chemical treatment by environment: metronidazole chemical treatment by environment: metronidazole
Observed In:	caudal hematopoietic tissue prostaglandin biosynthetic process myeloid cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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