ZFIN Image: Cacialli et al., 2022, Fig. 1

Image

Figure Caption

Fig. 1

The PGE₂ synthesis pathway in zebrafish CHT

A
Experimental outline of qPCR analysis after dissection of CHT (caudal region) at 48 hpf from kdrl:GFP, ikaros:GFP, mpeg1:GFP, mpx:GFP transgenic animals, and FACS‐sorted GFP‐positive cells to purify ECs, HSPCs, macrophages, and neutrophils.
B, C
The phospholipases pla2g4aa and pla2g4ab are highly expressed in neutrophils.
D–F
The expression of cyclooxygenases cox1, cox2a, and cox2b is enriched in macrophages.
G, H
The prostaglandin synthases ptges3a and ptges3b are only expressed by ECs.
I–M
The prostaglandin receptors ptger1a, ptger1b, ptger2a, ptger3, and ptger4a are mostly expressed in HSPCs.
N, O
The prostaglandin transporters slco2b1 and abcc4 are specifically expressed in ECs.

Data information: For each panel, data represent biological triplicates plated in technical duplicates. Statistical analysis was completed using one‐way ANOVA and multiple comparison tests. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Center values denote the mean, and error bars denote s.e.m.

Source data are available online for this figure.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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