UPR pathway mediates HSPC failure in dhx15?/? zebrafish. A, qRT?PCR analysis of CHOP, JUN, and ATF3 in dhx15+/+ and dhx15?/? zebrafish at 3 dpf. Each qRT?PCR experiment was carried out in triplicates, and repeated at least three times. B?E, WISH analysis of cmyb in dhx15+/+ and dhx15?/? zebrafish treated with 4?PBA. P < .005, ?2 test. F?I, WISH analysis of cmyb in dhx15+/+ and dhx15?/? zebrafish treated with toyocamycin. P < .005, ?2 test. Black arrow indicates caudal hematopoietic tissue. ***P < .0005. ATF3, activating transcription factor 3; dpf, days post fertilization; HSPC, hematopoietic stem/progenitor cell; qRT?PCR, quantitative real?time PCR; UPR, unfolded protein response pathway; WISH, whole?mount in situ hybridization; 4?PBA, 4?phenylbutyric acid
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