FIGURE

Fig. 4

ID

ZDB-FIG-221018-226

Publication

Cai et al., 2021 - Dhx15 regulates zebrafish definitive hematopoiesis through the unfolded protein response pathway

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Fig. 4

UPR pathway mediates HSPC failure in dhx15^−/− zebrafish. A, qRT‐PCR analysis of CHOP, JUN, and ATF3 in dhx15^+/+ and dhx15^−/− zebrafish at 3 dpf. Each qRT‐PCR experiment was carried out in triplicates, and repeated at least three times. B‐E, WISH analysis of cmyb in dhx15^+/+ and dhx15^−/− zebrafish treated with 4‐PBA. P < .005, χ² test. F‐I, WISH analysis of cmyb in dhx15^+/+ and dhx15^−/− zebrafish treated with toyocamycin. P < .005, χ² test. Black arrow indicates caudal hematopoietic tissue. ***P < .0005. ATF3, activating transcription factor 3; dpf, days post fertilization; HSPC, hematopoietic stem/progenitor cell; qRT‐PCR, quantitative real‐time PCR; UPR, unfolded protein response pathway; WISH, whole‐mount in situ hybridization; 4‐PBA, 4‐phenylbutyric acid

Expression Data

Genes:	atf3 ddit3 jun myb
Fish:	AB dhx15^{zf3775/zf3775} (AB)
Conditions:	chemical treatment by environment: 4-phenylbutyric acid chemical treatment by environment: toyocamycin
Anatomical Terms:	caudal hematopoietic tissue whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dhx15^{zf3775/zf3775} (AB)
Conditions:	chemical treatment by environment: 4-phenylbutyric acid chemical treatment by environment: toyocamycin
Observed In:	caudal hematopoietic tissue whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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