Fig. 8
SHOX/shox prevents chondrocyte senescence in WS models.
a?f Representative flow cytometry plots of three independent biological experiments for senescent analysis among CTR, shWRN1#, and rescue groups in hESC (a?c) and hMSC (d?f). g?j qRT-PCR measurement of P53 and P16Ink4a in CTR, shWRN1#, and rescue groups in hESC (g?h) and hMSC (i?j) on day 14. N = 3 independent biological experiments. k?m Representative FISH analysis of 3 independent experiments of p53 and p16 in WT, wrn?/? mutant, and rescue groups on 3 dpf, 7 dpf, and 14 dpf. Scale bar = 50 µm. Data are presented as the mean ± S.D. Statistical analysis was performed using two-tailed unpaired Student?s t-test. *P < 0.05, **P < 0.01, ***P < 0.001. |
Genes: | |
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Fish: | |
Anatomical Term: | |
Stage Range: | Protruding-mouth to Days 14-20 |
Fish: | |
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Observed In: | |
Stage Range: | Protruding-mouth to Days 14-20 |