FIGURE

Fig. 7

ID

ZDB-FIG-220908-29

Publication

Ma et al., 2022 - Ercc2/Xpd deficiency results in failure of digestive organ growth in zebrafish with elevated nucleolar stress

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Fig. 7

Ercc2/Xpd-deficient phenotypes are independent of an activated p53 response

(A) qPCR analysis of expressions of genes associated with tp53 activation in ercc2/xpd mutants and siblings at 5 and 7 dpf. Data are presented as mean ± SD from three independent biological repeats. Student’s t test, ∗, p < 0.05, ∗∗, p < 0.01, NS, non-significant.

(B) Whole-mount in situ hybridization showed a large up-regulation of tp53 in digestive organs (white arrowheads), retina (white arrow) and arches (black arrowheads) of ercc2/xpd mutants at 5 dpf. Scale bars, 100 μm.

(C) Whole-mount in situ hybridization revealed that tp53 mutation did not affect ercc2/xpd mutant phenotypes in digestive organs or hematopoiesis. Scale bars, 100 μm.

(D) Schematic diagram showing the possible mechanism of how Ercc2/Xpd deficiency leads to failure of digestive organ growth. See also Figure S9.

Expression Data

Genes:	fabp2 hbae3 mdm2
Fish:	ercc2^{whu312/whu312} ercc2^whu312/+ tp53^zdf1/zdf1; ercc2^whu312/+ tp53^zdf1/zdf1; ercc2^{whu312/whu312}
Anatomical Terms:	digestive system whole organism
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ercc2^{whu312/whu312} ercc2^{whu312/whu312} tp53^zdf1/zdf1; ercc2^whu312/+ tp53^zdf1/zdf1; ercc2^{whu312/whu312}
Observed In:	digestive system swim bladder whole organism
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

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