FIGURE

Fig. 4

ID

ZDB-FIG-220718-20

Publication

Li et al., 2022 - Estrogens revert neutrophil hyperplasia by inhibiting Hif1α-cMyb pathway in zebrafish myelodysplastic syndromes models

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

E2 decreases c-myb expression in c-myb^hyper transgenic zebrafish embryos.

A E2 exposure decreased c-myb in the CHT, as determined by WISH. B qPCR quantification of the decreased c-myb expression by E2 (the c-myb^hyper fusion gene contained two parts, which comprised truncated c-myb from exon1 to exon10, followed by a near full-length c-myb from exon2 to exon 15). c-myb-primer1 and c-myb-primer2 were designed at repetitive and nonrepetitive sequences. (t-test, mean?�?SEM. ***p?<?0.001, **p?<?0.01, n?>?10). C qPCR quantification of decreased c-myb expression in c-myb^hyper adult zebrafish kidney after treatment with E2. (t-test, mean?�?SEM. ^***p?<?0.001, ^*p?<?0.05, n?>?10) (D) FACS analysis confirmed that E2 diminished c-myb:GFP⁺ cells. (t-test, mean?�?SEM. ^*p?<?0.05, n?>?10) (E) qPCR quantification of decreased c-myb expression in c-myb:GFP⁺ cells by E2. (t-test, mean?�?SEM. ***p?<?0.001, n?>?10). F qPCR quantification of decreased c-myb expression in lyz:Dsred⁺ cells by E2. (t-test, mean?�?SEM. ***p?<?0.001, **p?<?0.01, n?>?10).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Discov