ZFIN Image: Li et al., 2022, Fig. 4

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Fig. 4

E2 decreases c-myb expression in c-myb^hyper transgenic zebrafish embryos.

A E2 exposure decreased c-myb in the CHT, as determined by WISH. B qPCR quantification of the decreased c-myb expression by E2 (the c-myb^hyper fusion gene contained two parts, which comprised truncated c-myb from exon1 to exon10, followed by a near full-length c-myb from exon2 to exon 15). c-myb-primer1 and c-myb-primer2 were designed at repetitive and nonrepetitive sequences. (t-test, mean ± SEM. ***p < 0.001, **p < 0.01, n > 10). C qPCR quantification of decreased c-myb expression in c-myb^hyper adult zebrafish kidney after treatment with E2. (t-test, mean ± SEM. ^***p < 0.001, ^*p < 0.05, n > 10) (D) FACS analysis confirmed that E2 diminished c-myb:GFP⁺ cells. (t-test, mean ± SEM. ^*p < 0.05, n > 10) (E) qPCR quantification of decreased c-myb expression in c-myb:GFP⁺ cells by E2. (t-test, mean ± SEM. ***p < 0.001, n > 10). F qPCR quantification of decreased c-myb expression in lyz:Dsred⁺ cells by E2. (t-test, mean ± SEM. ***p < 0.001, **p < 0.01, n > 10).

Acknowledgments

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