FIGURE

Fig. 5

ID

ZDB-FIG-220601-12

Publication

Lelieveld et al., 2022 - Consequences of excessive glucosylsphingosine in glucocerebrosidase-deficient zebrafish

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Fig. 5

Fig. 5. Biochemical abnormalities in adult zebrafish brain. A: Lipid levels were determined of dissected brains in pmol/mg tissue. GlcSph, GalCer, and GlcChol were separated from their respective galactosylated lipid by HILIC chromatography. Data is depicted as mean ± SEM; WT (n = 9–10), gba1^-/- (n = 9), asah1b^-/- (n = 8), and gba1^-/-:asah1b^-/- (n = 11). Data is analyzed by One-Way Anova with Tukey’s multiple comparison test. B: mRNA levels of asah1a, asah1b, gpnmb, chia.6, il-1β, tnfβ, apoEb, catD, c1qA, c3.1, c5aR, and c5 was determined using RT-qPCR analysis; WT (n = 9–11), gba1^-/- (n = 8–11), asah1b^-/- (n = 5), and gba1^-/-:asah1b^-/- (n = 9–10). Data is analyzed by One-way Anova with Tukey’s multiple comparison test. C: Representative Western blot of p62, LC3-I, LC3-II, β-catenin protein levels in WT, gba1^-/-, asah1b^-/-, and gba1^-/-:asah1b^-/- zebrafish brains, with β-actin as protein loading control. D: Quantitative analysis of LC3-II/LC-I levels and p62 protein levels. WT (n = 5), gba1^-/- (n = 5), asah1b^-/- (n = 3), and gba1^-/-:asah1b^-/- (n = 6). Statistical analysis is depicted of WT versus gba1^-/-, asah1b^-/-, or asah1b^-/-:gba1^-/-, only when a significant difference is apparent or of gba1^-/- versus asah1b:gba1^-/- when significant difference is of interest. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗∗ p < 0.0001. GalCer, galactosylceramide; GlcSph, glucosylsphingosine; GlcChol, glucosylated cholesterol.

Expression Data

Genes:	apoeb asah1a asah1b c1qa c3a.1 c5 c5ar1 chia.6 gpnmb il1b tnfb
Antibodies:	Ab2-map1lc3 Ab9-sqstm1
Fish:	AB/TL asah1b^{zf3576/zf3576} (AB/TL) gba1^{zf3252/zf3252} (AB/TL) asah1b^{zf3576/zf3576}; gba1^{zf3252/zf3252} (AB/TL)
Anatomical Term:	brain
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	asah1b^{zf3576/zf3576} (AB/TL) gba1^{zf3252/zf3252} (AB/TL) asah1b^{zf3576/zf3576}; gba1^{zf3252/zf3252} (AB/TL)
Observed In:	brain brain autophagy brain ceramide brain cholesteryl glycoside brain D-glucosylsphingosine brain galactosylceramide brain glucosylceramide brain Lactosylceramide (d18:1/12:0)
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Lipid Res.