FIGURE

Fig. 3

ID

ZDB-FIG-220601-10

Publication

Lelieveld et al., 2022 - Consequences of excessive glucosylsphingosine in glucocerebrosidase-deficient zebrafish

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Fig. 3

Fig. 3. (Glyco)sphingolipid abnormalities in Asah1a- and/or Asah1b-deficient zebrafish larvae. A: Total ceramide levels (pmol/fish) were determined of individual zebrafish larvae (5 dpf) of off-spring of Asah1a^+/-:asah1b^+/- crossings. Data is depicted as mean ± SD and analyzed using a One-Way Anova with Dunnett’s multiple comparisons Test and WT as control group. B: Asah1a^+/-:asah1b^+/- adult zebrafish were crossed and off-spring was treated with vehicle (0.1% (v/v) DMSO) or 10 μm GCase specific inhibitor (ME656) for 5 days. Relevant lipid levels were determined of individual larvae in pmol/fish. WT (n = 5–6), asah1a^-/-:asah1b^+/- (n = 1–5), asah1a^+/+:asah1b^-/- (n = 6), asah1^+/-:asah1b^-/- (n = 7–8), and asah1a^-/-:asah1b^-/- (n = 4–8). Data is depicted as mean ± SD and analyzed using a Two-Way Anova with Sidak’s multiple comparisons test. Ns = not significant, ∗p < 0.05,∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL asah1a^{zf3575/zf3575} (AB, AB/TL) asah1b^{zf3576/zf3576} (AB/TL) asah1a^zf3575/+; asah1b^{zf3576/zf3576} (AB/TL) asah1a^{zf3575/zf3575}; asah1b^zf3576/+ (AB/TL) asah1a^{zf3575/zf3575}; asah1b^{zf3576/zf3576} (AB/TL)
Condition:	chemical treatment by environment: EC 3.2.1.45 (glucosylceramidase) inhibitor
Observed In:	whole organism ceramide whole organism cholesteryl glycoside whole organism D-glucosylsphingosine whole organism HexCer(d34:1)
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Lipid Res.