FIGURE
Figure 7
- ID
- ZDB-FIG-220430-150
- Publication
- Wu et al., 2022 - Slc38a9 Deficiency Induces Apoptosis and Metabolic Dysregulation and Leads to Premature Death in Zebrafish
- Other Figures
- All Figure Page
- Back to All Figure Page
Figure 7
Figure 7. Slc38a9 deficiency leads to dysregulated hypoxia response. (A) The FPKM of hypoxia-induced genes in WT and slc38a9 mutant zebrafish embryos (60 hpf). (B) The phenotypic penetrance of the slc38a9 mutant increased under hypoxia treatment. Zebrafish larvae (24 hpf) were treated with hypoxia embryo rearing solution (DO = 1 mg/L) for 10 h. Normoxia (Nor) and hypoxia (Hyp). (C) The hypoxia-induced increase of Hif1α target genes was blocked in the slc38a9 mutant zebrafish. The experimental groups were the same as described in (B). The expression of Hif1α target genes was detected by qRT-qPCR and normalized by the β-actin mRNA levels. The mRNA levels of Hif1α target genes were upregulated in WT zebrafish larvae under hypoxic conditions, while this increase was completely blocked in the slc38a9 mutant. Data shown are mean ± SEM, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001. (D) The increase of Hif1α protein level was attenuated in slc38a9 mutants under hypoxic conditions. The experimental groups were the same as described in (B). The immunoprecipitation was used to enrich the Hif1α protein. The representative result is shown in (D). > Hif1α.
|
Expression Data
| Genes: | |
|---|---|
| Antibody: | |
| Fish: | |
| Condition: | |
| Anatomical Term: | |
| Stage: | Pec-fin |
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Condition: | |
| Observed In: | |
| Stage: | Pec-fin |
Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Int. J. Mol. Sci.