FIGURE

FIGURE 4

ID

ZDB-FIG-220424-4

Publication

Ouyang et al., 2022 - An EGFP Knock-in Zebrafish Experimental Model Used in Evaluation of the Amantadine Drug Safety During Early Cardiogenesis

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FIGURE 4

CPAAHTs exposure suppressed the expression of genes required for myocardium differentiation and LR asymmetry. (A,B) Dorsal view of WISH images (A) and quantitative RT-PCR analysis (B) of transcription factors nkx2.5, gata4, mef2ca, and tbx20 in 0.8 μg/μl CPAAHTs treated embryos compared with untreated controls at 8-somite stage (SS). Arrows point to expected expression patterns for the corresponding genes. N = 3. Unpaired 2-tailed Student’s t-test. (C,D) Dorsal view of WISH images (C) and quantitative RT-PCR analysis (D) of Nodal-related gene spaw and its downstream target genes in 0.8 μg/μl CPAAHTs treated embryos compared with untreated controls at 25- to 28-SS. Arrows point to the expected expression patterns for the corresponding genes. N = 3. Unpaired 2-tailed Student’s t-test.

Expression Data

Genes:	gata4 lft1 lft2 mef2ca nkx2.5 pitx2 spaw tbx20
Fish:	myh7^hn2Tg/hn2Tg
Condition:	chemical treatment by environment: amantadine
Anatomical Terms:	lateral plate mesoderm presumptive cardiac ventricle primitive heart tube whole organism
Stage Range:	5-9 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	myh7^hn2Tg/hn2Tg
Condition:	chemical treatment by environment: amantadine
Observed In:	lateral plate mesoderm presumptive cardiac ventricle primitive heart tube whole organism
Stage Range:	5-9 somites to 26+ somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cardiovasc Med