ZFIN Image: Ouyang et al., 2022, FIGURE 4

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Figure Caption

FIGURE 4

CPAAHTs exposure suppressed the expression of genes required for myocardium differentiation and LR asymmetry. (A,B) Dorsal view of WISH images (A) and quantitative RT-PCR analysis (B) of transcription factors nkx2.5, gata4, mef2ca, and tbx20 in 0.8 μg/μl CPAAHTs treated embryos compared with untreated controls at 8-somite stage (SS). Arrows point to expected expression patterns for the corresponding genes. N = 3. Unpaired 2-tailed Student’s t-test. (C,D) Dorsal view of WISH images (C) and quantitative RT-PCR analysis (D) of Nodal-related gene spaw and its downstream target genes in 0.8 μg/μl CPAAHTs treated embryos compared with untreated controls at 25- to 28-SS. Arrows point to the expected expression patterns for the corresponding genes. N = 3. Unpaired 2-tailed Student’s t-test.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cardiovasc Med