Figure 3

Hmx1^mut10 embryos present enhanced expression of osteogenic factors: RT-PCR analysis of the BMP antagonist chordin and noggin1 relative to β-actin, the osteogenic inducers bmp2b and bmp4, and the osteogenic markers runx2b and spp1. In hmx1^mut10 embryos, chordin expression is reduced at 2 dpf (A), while noggin1 expression is lower at 1 dpf and 3 dpf (B). Bmp2b and bmp4 transcripts are significantly different in hmx1^mut10 embryos when compared to wildtype zebrafish from 1 dpf to 3 dpf (C,D). The osteogenic markers runx2b and spp1 are differentially expressed, and are higher in hmx1^mut10 at 2 dpf and 3 dpf, respectively (E,F). Data are expressed as mean of three or more experiments.