FIGURE

Figure 4.

ID

ZDB-FIG-220404-24

Publication

Wiggin et al., 2022 - V3 Interneurons are Active and Recruit Spinal Motor Neurons During In Vivo Fictive Swimming in Larval Zebrafish

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Figure 4.

V3-IN activity is temporally correlated to fictive locomotion. A, Schematic representing the number and distribution of V3-INs (green circles) along the rostrocaudal axis of the spinal cord monitored during calcium imaging experiments. B, Each V3-IN in the field of view in Tg(vglut2a:Gal4ff)^nns20;Tg(UAS:GCaMP6s)^nk13a double transgenic larvae was assigned a ROI during spontaneous fictive swimming. Panels in B show raw GCaMP6s fluorescence (F) and correspond to numbered time points in C. Bottom panel in B shows calculated ΔF/F between 3- and 7-s time points. Dashed lines represent ventral boundary of the spinal cord. C, Synchronized recordings of GCaMP6s fluorescence (top) and fictive swimming (bottom) allowed identification of swimming-related neuronal activity. D, Plots of cumulative probability distributions for Mean (black line) and Peak (gray line) burst frequencies.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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