FIGURE

Fig. 5

ID

ZDB-FIG-220319-24

Publication

Wen et al., 2022 - Loss of Ripk3 attenuated neutrophil accumulation in a lipopolysaccharide-induced zebrafish inflammatory model

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Fig. 5

Deficiency of ripk3 attenuated the LPS-induced inflammatory responses.

A Experimental design. The 3 dpf WT and ripk3-deficient larvae were injected with LPS, and collected at 5 hpi for the detection of cytokines. The neutrophil accumulation was evaluated by SB staining at 2, 5, and 8 hpi. B Fewer neutrophils migrated to the injection sites. At 2, 5, and 8 hpi, fewer neutrophils (red arrows pointed) could be found in the yolk sac of the ripk3-deficient larvae. C Quantification of (B) (n ≥ 6, one-way ANOVA). D Quantification of (B), showing the trends of the accumulated neutrophils. E The expression levels of pro-inflammatory cytokines (il1b, il6, and cxcl8a) at 5 hpi (n ≥ 10, one-way ANOVA). F The expression levels of anti-inflammatory cytokines (il4, il10, and il13) at 5 hpi (n ≥ 10, one-way ANOVA).

Expression Data

Genes:	cxcl8a il1b il4 il6 il10 il13
Fish:	AB ripk3^szy12/szy12
Condition:	chemical treatment by injection: lipopolysaccharide
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB ripk3^szy12/szy12
Condition:	chemical treatment by injection: lipopolysaccharide
Observed In:	neutrophil whole organism yolk
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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