FIGURE

Fig 1

ID

ZDB-FIG-220214-19

Publication

Hamilton et al., 2022 - Enteric nervous system modulation of luminal pH modifies the microbial environment to promote intestinal health

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Fig 1

sox10 mutants have decreased intestinal transit and hyperpermeability, independent of intestinal microbially induced inflammation.

Representative bright field and fluorescence microscopy images showing (A) WT, and (B) sox10^-/- larvae (top), dissected intestines stained for myeloperoxidase activity to reveal intestinal neutrophils (middle) and after oral microgavage of 4 kD fluorescein dextran (FD4) (bottom). Arrows indicate intestinal neutrophils. (C) Quantification of intestinal neutrophils per mid and distal intestine in CV and GF larvae. (D) Quantification of intestinal transit of phenol red; distance traveled over time measured as somites/hour (GF WT vs GF sox10^-/- t-test p = 0.02). Quantification of (E) FD4 and (F) 4 kD tetramethylrhodamine dextran (TD4) permeability measured as fluorescence intensity in aorta. Scale bars = 300μm. AU = arbitrary units. Each dot in C-F is one fish; n>18 for each condition. Boxes in C-F represent the first to third quartiles, center bar denotes the median, and whiskers the maximum and minimum of each dataset. * p < 0.05, **p<0.01, ****p<00001. A and B: ANOVA followed by Tukey’s post hoc test, C and D: Kolmogorov-Smirnov non-parametric t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sox10^t3/t3 sox10^t3/+
Condition:	germ free
Observed In:	gut establishment of endothelial intestinal barrier gut neutrophil gut neutrophil migration
Stage:	Day 6

Phenotype Detail

Acknowledgments

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