Fig 4

(A) Top left, representative images of WT (top) and sox10 mutant (bottom) larvae 20 min after m-cresol purple gavage. Bottom, pictures of indicator dyes in sterile embryo medium adjusted to known pHs; graph shows quantification of standards. (B) Quantification of luminal pH in intestinal bulb plotting red + green–blue integer values as described in the Methods. (C) Top left, representative images of WT (top) and sox10 mutant (bottom) larvae 20 min after phenol red gavage. Bottom, pictures of sterile embryo medium adjusted to known pHs; graph shows quantification of standards. (D) Quantification of luminal pH in intestinal bulb plotting red integer values. Integer values correspond with red, green and blue channel pixel intensities between 0 and 255 identified with “RGB measure” Image J plugin (see Methods). (A&C) Each dot is an average of at least 3 replicates of pH standard values. Black circle indicates value of WT representative image and red dot indicates value of sox10^-/- representative image. (B&D) Each dot is an individual fish; n>17 for each condition. Boxes represent the first to third quartiles, center bar denotes the median, and whiskers the maximum and minimum of each dataset. * p < 0.05, ****p<00001. ANOVA followed by Tukey’s post hoc test.