Figure 3

Figure 3. RNA-seq analysis of 6dpf panx1b^−/− larvae. (a) Comparison of differentially regulated RNA transcripts against two categories: central nervous system (CNS) and the visual system. (b) GO annotation of RNA-seq data in terms of biological process (BP): the upregulated RNA transcripts were enriched in the regulation of metabolic processes, whereas the downregulated genes were annotated for RNA processing, circadian rhythms, amino-acid metabolic process, translation, negative regulation of nitrogen (N) compounds metabolism, and G-protein coupled receptor signaling. (c) Validation of RNA-seq data by RT-qPCR. The significant downregulation of cry1, cry5, bmal1a, bmal1b, and clock2 genes in 6 dpf panx1b^−/− larvae was confirmed by RT-qPCR in n = 3 independent replicates. (d) Cartoon illustrating the network of circadian clock genes differently regulated in panx1b^−/− larvae. Regulated genes in bold. (e) Illustration of the connection of the inner retina with optic tectum, habenula (Ha), and pretectum (p) conferring light sensitivity and circadian rhythmicity, and descending control of locomotion. Abbreviation: nMLF, nucleus of the medial longitudinal fasciculus.