FIGURE

FIGURE 3

ID

ZDB-FIG-211118-3

Publication

Xu et al., 2021 - Overexpression of Lifeact-GFP Disrupts F-Actin Organization in Cardiomyocytes and Impairs Cardiac Function

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FIGURE 3

The effect of Lifeact-GFP expression on actin filament and sarcomere organization in cardiomyocytes of Tg(myl7:Lifeact-GFP)^mb21 and Tg(myl7:Lifeact-GFP)^mb22 transgenic embryos. (A–F) Actin think filaments revealed by Lifeact-GFP (A,D) and phalloidin staining (B,E) in Tg(myl7:Lifeact-GFP)^mb21 (A–C), and Tg(myl7:Lifeact-GFP)^mb22 (D–F) transgenic embryos at 72 hpf. (C,F) are merged pictures of A-B and D-E, respectively. (G–L) The sarcomeric organization of actin think filament and Z-lines revealed by Lifeact-GFP (G,J) anti-α-actinin antibody staining (H,K) in Tg(myl7:Lifeact-GFP)^mb21 (G–I), and Tg(myl7:Lifeact-GFP)^mb22 (J–L) transgenic embryos at 72 hpf. (I,L) are merged pictures of (G,H,J,K), respectively. Scale bars: 25 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol