FIGURE

FIGURE 4

ID

ZDB-FIG-211118-4

Publication

Xu et al., 2021 - Overexpression of Lifeact-GFP Disrupts F-Actin Organization in Cardiomyocytes and Impairs Cardiac Function

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FIGURE 4

Identification of transgene integration sites in Tg(myl7:Lifeact-GFP)^mb22 and Tg(myl7:Lifeact-GFP)^mb23 transgenic embryos and the rescue of heart defect by knockdown of Lifeact-GFP expression. (A,B) Mapping the transgene integration sites in Tg(myl7:Lifeact-GFP)^mb22 and Tg(myl7:Lifeact-GFP)^mb23 transgenic embryos at cadherin2 and traf4a genes, respectively. The Lifeact-GFP-MO is indicated by the red line. (C–F) Morphology of heart region of Tg(myl7:Lifeact-GFP)^mb22 transgenic embryos at 72 hpf injected with control-MO (C,D) and Lifeact-GFP-MO (E,F), respectively. Pericardial edema is indicated by the arrow in control-MO injected Tg(myl7:Lifeact-GFP)^mb22 (D) transgenic mutant larvae. H, heart; Yolk shows autofluorescence. Scale bars: 250 μm. (G–L) Lifeact-GFP expression (G,J) and phalloidin staining (H,K) showing actin think filaments in cardiomyocytes of Tg(myl7:Lifeact-GFP)^mb22 transgenic embryos injected with control-MO (G–I) or Lifeact-GFP-MO (J–L) at 72 hpf transgenic embryos. (I,L) are merged pictures of (G,H,J,K), respectively. Scale bars: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol