FIGURE

Fig 7

ID

ZDB-FIG-211116-16

Publication

Bühler et al., 2021 - Histone deacetylase 1 controls cardiomyocyte proliferation during embryonic heart development and cardiac regeneration in zebrafish

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Fig 7

Hdac1 is involved in heart regeneration after cryoinjury.

(A, B) Experimental timeline of cryoinjury, treatment and harvesting of the hearts for the analysis of cardiomyocyte proliferation (A) revascularization (4 dpi) and scar formation (30 dpi) (B). (C, D) Immunohistochemistry for mCherry (red, cardiomyocytes) and PCNA (green) on 7 dpi hearts of Tg(myl7:NLS-mCherry) fish treated with either DMSO or Mocetinostat from 1 to 7 dpi, showed reduced rate of cycling cardiomyocytes at the wound border zone in Mocetinostat-treated hearts (% of PCNA⁺/mCherry⁺ cardiomyocytes DMSO 9.91 ± 2.14, n = 12, Mocetinostat 6.1 ± 2.96, n = 11, p = 0.0018). (E, F) Native GFP (green, endothelial cells) and mCherry (red, cardiomyocytes) fluorescence in 4 dpi hearts of Tg(fli:GFP^y1;myl7:NLS-mCherry) fish treated with DMSO or Mocetinostat from 12 hpi to 4 dpi, showed no changes in revascularization (% of GFP⁺ wound area: DMSO Ctrl 16.26 ± 6.34, n = 7, Mocetinostat 15.74 ± 5.62, n = 8, p > 0.9999). (G, H) Acid Fuchsin Orange G (AFOG) staining of 30 dpi hearts treated with DMSO or Mocetinostat from 1 to 30 dpi, have no significant difference in wound size (DMSO 4.05 ± 4.85, n = 8, Mocetinostat 5.59 ± 2.91, n = 8, p = 0.3282). Error bars indicate s.d.; **p < 0.01, ns, not significant. Scale bars, 100 μm.

Expression Data

Genes:	EGFP mCherry
Antibody:	Ab2-pcna
Fish:	ulm106Tg (AB) ulm106Tg; y1Tg (AB)
Conditions:	cold damage: cardiac ventricle chemical treatment by environment: mocetinostat, cold damage: cardiac ventricle
Anatomical Terms:	cardiac muscle cell cardiac muscle myoblast heart blood vessel endothelial cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB ulm106Tg (AB) ulm106Tg; y1Tg (AB)
Condition:	chemical treatment by environment: mocetinostat, cold damage: cardiac ventricle
Observed In:	cardiac muscle cell cell population proliferation cardiac ventricle cardiac muscle tissue regeneration cardiac ventricle vasculogenesis
Stage:	Adult

Phenotype Detail

Acknowledgments

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