FIGURE

Fig 6

ID

ZDB-FIG-211116-15

Publication

Bühler et al., 2021 - Histone deacetylase 1 controls cardiomyocyte proliferation during embryonic heart development and cardiac regeneration in zebrafish

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Fig 6

Mocetinostat treatment of zebrafish embryos induces the bal phenotype.

(A) Survival rate of Mocetinostat- and Parthenolide-treated embryos, compared to the DMSO control. At 24 hpf (DMSO: 99.0 ± 2.0%; Mocetinostat: 96.0 ± 3.27%; Parthenolide: 86.0 ± 9.52%, n = 4), at 48 hpf (DMSO: 98.0 ± 4.0%; Mocetinostat: 93.0 ± 3.83%; Parthenolide: 61.0 ± 10.0%, n = 4) and at 72 hpf (DMSO: 97.0 ± 3.83%; Mocetinostat: 87.0 ± 6.83%; Parthenolide: 11.0 ± 10.0%, n = 4). (B-D) Lateral view of inhibitor- or DMSO-treated embryos at 72 hpf. (E) Western blot analysis of acetylation levels of Histone 3 (H3ac) and 4 (H4ac) of lystes from Mocetinostat and DMSO-treated embryos, show a pronounced hyperacetylation of H3 and H4 in Mocetinostat-treated embryos. pan Histone 3 served as a loading control. (F-G) IF staining against meromyosin (MF20) (red) and atrial-specific myosion (S46) (green) demonstrated normal chamber specification of Mocetinostat-treated hearts. (H-J) Number of ventricular cardiomyocytes was reduced in Mocetinostat treated Tg(myl7:mCherry) embryos (DMSO: 197.0 ± 17.39; Mocetinostat: 170.4 ± 7.7, n = 5). (K-M) The rate of ventricular pH3⁺ cardiomyocytes was reduced in Mocetinostat-treated embryos as well (DMSO: 2.1 ± 0.48%; Mocetinostat: 0.8± 0.30%, n = 5). Error bars indicate s.d., *p < 0.05, **p < 0.01, ****p < 0.0001.

Expression Data

Gene:	mCherry
Antibodies:	Ab-MF20 Ab-S46 Ab2-h3 Ab13-h4
Fish:	AB ulm106Tg (AB)
Condition:	chemical treatment by environment: mocetinostat
Anatomical Terms:	atrium cardiac muscle cell cardiac ventricle whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB ulm106Tg (AB)
Conditions:	chemical treatment by environment: mocetinostat chemical treatment by environment: parthenolide
Observed In:	atrium cardiac muscle cell differentiation cardiac muscle cell cell population proliferation cardiac ventricle cardiac ventricle cardiac muscle cell cardiac ventricle cardiac muscle cell differentiation whole organism
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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